1.
Genet. mol. biol
;
40(3): 597-599, July-Sept. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-892428
ABSTRACT
Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).